Coding

Part:BBa_K363009:Design

Designed by: Justin Porter   Group: iGEM10_Johns_Hopkins   (2010-10-26)

Mid1, votage and stretch gated calcium channel from S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene is a canonical gene from the S. cerevisiae genome, except that it has had the restriction sites for EcoRI, SpeI, XbaI, and BgIII removed. It has been otherwise codon optimized for yeast.


Source

This part comes from the fourteenth chromosome of the baker's yeast Saccharomyces cerevisiae.

References

  • Iida H, et al. MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating. Mol Cell Biol (1994)
  • Peiter E, et al. The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. FEBS Lett (2005)