Coding
Part:BBa_K363009:Design
Designed by: Justin Porter Group: iGEM10_Johns_Hopkins (2010-10-26)
Mid1, votage and stretch gated calcium channel from S. cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This gene is a canonical gene from the S. cerevisiae genome, except that it has had the restriction sites for EcoRI, SpeI, XbaI, and BgIII removed. It has been otherwise codon optimized for yeast.
Source
This part comes from the fourteenth chromosome of the baker's yeast Saccharomyces cerevisiae.
References
- Iida H, et al. MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating. Mol Cell Biol (1994)
- Peiter E, et al. The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. FEBS Lett (2005)